Wednesday, July 11, 2018

HPLC-05

Know the difference between UV/VIS, PDA and DAD detectors

 Don't think all these three detectors work on different principle. (refer HPLC-03 post for abbreviations) PDA and DAD are actually UV/VIS detectors and they have an array of photodiodes. Photodiode is used to convert light into electrical signal.The UV/Vis detector can detect  single wavelength at one time while the DAD/PDA can scan a wavelength range (200-800 nm). If you want to scan a wavelength range DAD/PDA are better or If you know the particular absorbance wavelength, go with UV/VIS dtector. So UV/VIS (PDA or DAD) are the most commonly used detectors in HPLC analysis because it is also very easy to analyse/interpret the peaks in the chromatogram.
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