Thursday, June 14, 2018

SEC-01

Size Exclusion Chromatography (SEC)

  • Used mainly to separate high molecular weight samples and to determine their molecular weight distribution by virtue of their size in solution. 
  • Also known as gel permeation, gel filtration or steric exclusion chromatography.
  • Stationary phase is made of beads with pores of defined diameter. 
  • Very large molecules can’t enter into the pores and so elute first, Medium sized molecule enter with difficulties and so elute second. Small molecules enter all pores  and elute as last.
Principle of SEC
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