Tuesday, July 24, 2018

HPLC-08

Know about Void volume and Dead volume

Void volume
The volume of mobile phase in the column is Void volume. If the stationary phase occupies 45% of total column volume, the void volume would be 55% of total column volume.

 Dead volume
The volume between the injection point and detection point excluding the stationary phase in the column. It is also known as Extra-column volume.
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