Why Column Temperature is the important factor in HPLC?
The maintenance of optimum temperature plays a vital role in the separation of analyte of interest from a sample. Temperature of column directly influences with the viscosity of the mobile phase. Increase in temperature of column decreases the viscosity of the mobile phase. Therefore the viscosity gets decreased, the flow rate becomes fast and it also reduces longitudinal diffusion. So, Optimum temperature (It shouldn't be very high or low) should be maintained for the better separation of peaks.
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