Saturday, June 30, 2018

General info-08

Guard columns

In HPLC, Guard columns are mostly used to extend the life of the analytical columns. Guard column is a small sized column packed with the same packing material as like analytical column. Since the frits on guard column also remains same as those on analytical column, the particles blocking the analytical column will block the guard column first. So the analytical column will be safeguarded by the use of guard columns.
(Analytical columns- Columns like IEX, RPC, SEC, HIC normally used for the purification and analysis of samples)
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