Sunday, August 5, 2018

HPLC -10

Relative Retention Time (RRT)

RRT is the ratio of retention time of peaks corresponding to the compounds other than analyte and the main peak retention time. For example, If the retention time of main peak is 8 minutes and the retention time of other impurity peak is 5 minutes. The RRT of impurity peak is 5/8=0.625 and the mainpeak RRT is 8/8=1.
RRT is mainly used to control the elution of impurity peaks from the sample.
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