Saturday, August 18, 2018

HPLC-14

Extra-Column band broadening

Extra-Column band broadening occurs due to more Extra-column volume or Dead volume (refer post HPLC-08). Tubing connections between column and injection system, column and detector region can cause band or peak broadening. Therefore larger the radius of connecting tube, more the extra-column peak broadening and smaller the radius of connecting tube, less the extra-column broadening.
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