Sunday, August 5, 2018

HPLC-11

Causes for the the change of Retention time

 The molecule of analyte in a sample has to elute at 8th minute. But instead, If it gets eluted either before or after that 8th minute there is a change in retention time. It happens due to some factors like variation of column temperature, evaporation of mobile phase, Improper preparation of mobile phase. So it is necessary to maintain optimum temperature in column and prepare the mobile phase accurately.
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