Friday, June 15, 2018

General Info-03

Why caffeine is mostly used for calibration of UV detectors in HPLC?

Caffeine gives response at two maximas: one at 203 nm, second at 273 nm & one minima at 245nm.

Since Methyl, Ethyl & Propyl Paraben  absorbs at two maximas and one minima, It is also used as a standard for calibration of UV detectors.
By at

No comments:

Post a Comment

Subscribe to: Post Comments (Atom)

HPLC-15

Column efficiency Column efficiency is the ability of the column to produce the good resolution and narrow peaks. Factors affecting Col...

  • IEX-02
    Stationary phase in IEX The cation exchanger has sulfomethyl, carboxymethyl groups and the anion exchanger has Quarternary ammonium, Di...
  • SEC-01
    Size Exclusion Chromatography (SEC) Used mainly to separate high molecular weight samples and to determine their molecular weight distri...
  • IEX-01
    Ion Exchange Chromatography (IEX)  Ion exchange chromatography (IEX) separates proteins based on their net surface charge, through elec...