Tuesday, June 19, 2018

RPC-01

Reverse Phase Chromatography (RPC)

Reverse Phase chromatography separates molecules based on the differences in hydrophobicity of molecules. RPC uses hydrophobic stationary phase. The hydrophobic nature is achieved by the non polar functional groups bonded to the stationary phase. The mobile phase in RPC is polar. The non-polar compounds bind to the stationary phase by hydrophobic interaction. Then the bonded analytes are eluted from the column by using non-polar solvents.(Mant C T, 1996)(Jason Olbrich, 2013)


Principle of Reverse Phase Chromatography
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