Know about Isoelectric Point or Isoionic point (pI)
One of the most probable Interview questions in many pharma industries
- The pH point at which protein no longer has a net charge. This doesn't mean that the molecule has no charge. It has charge i.e., all the charges are neutralized.
- we can also say pI is the pH at which the amino acid does not migrate in an electric field
- Ion exchange chromatography works mostly based on pI of the protein to be separated.
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