Ion Exchange Chromatography (IEX)
Ion exchange chromatography (IEX) separates proteins based on their net surface charge, through electrostatic interactions that occur between proteins and a charged stationary phase.
Types of IEX
Two types of IEX exist:
1) Anion exchange (Negatively charged proteins bind to positively charged stationary phase)
2) Cation exchange (Positively charged proteins bind to negatively charged stationary phase)
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