Thursday, June 28, 2018

IEX-03

Effect of Isoelectric point on IEX

Every protein has an Isoelectric point (pI) (refer General info-01). When a protein is at its pI the protein will not even bind to the ion-exchange resin. Below this pH the protein will have a net positive charge and will bind to a cation exchanger, and above this pH it will have a net negative charge and bind to an anion exchanger. 
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