Thursday, June 21, 2018

General info-06

Modes of elution

Isocratic elution - The mobile phase composition remains the same throughout the run time. 
Example: Don't think only 100%  of  mobile phase A or B is Isocratic mode. If the run time is 8 minutes, either the mobile phase of 100% or  composition of A-80% + B-20%  runs same for 8 minutes in this mode.

Gradient elution - The mobile phase composition changes during the run time. 
Example: If the run time is same 8 minutes, the mobile phase composition of A-80% + B-20% runs for first 4 minutes and it changes to A-60% + B-40% for next 4 minutes in gradient mode.

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